Osterman, A. L. & Gerdes, S. Y. ed.
Microbial Gene Essentiality
Protocols and Bioinformatics
Humana Press 2008.3
486 pp.(H)
ISBN 1-58829-378-5
14,800円
Contents
Part I Experimental Protocols: 1 Overview of Whole-Genome Essentiality Analysis/ 2 Transposon-Based Strategies for the Identifi cation of Essential Bacterial Genes/ 3 Identifi cation and Analysis of Essential Genes in Haemophilus infl uenzae/ 4 Transposon Site Hybridization in Mycobacterium tuberculosis/ 5 Essential Genes in the Infection Model of Pseudomonas aeruginosa PCR-Based Signature-Tagged Mutagenesis/ 6 Whole-Genome Detection of Conditionally Essential and Dispensable Genes in Escherichia coli via Genetic Footprinting/ 7 Generating a Collection of Insertion Mutations in the Staphylococcus aureus Genome Using bursa aurealis/ 8 Multipurpose Transposon Insertion Libraries for Large-Scale Analysis of Gene Function in Yeast/ 9 How to Make a Defi ned Near-Saturation Mutant Library/ 10 Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality/ 11 The Construction of Systematic In-Frame, Single-Gene Knockout/ 12 The Applications of Systematic In-Frame, Single-Gene Knockout/ 13 A Novel, Simple, High-Throughput Method for Isolation of Genome- Wide Transposon Insertion Mutants of Escherichia coli K-12/ 14 High-Throughput Creation of a Whole-Genome Collection of Yeast Knockout Strains/ 15 Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray/ 16 Scarless Engineering of the Escherichia coli Genome/ 17 Minimization of the Escherichia coli Genome Using the Tn5-Targeted Cre/loxP Excision System/ 18 Construction of Long Chromosomal Deletion Mutants of Escherichia coli and Minimization of the Genome/ 19 Identifi cation of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library/ 20 Techniques for the Isolation and Use of Conditionally Expressed Antisense RNA to Achieve Essential Gene Knockdowns in Staphylococcus aureus/ 21 Introduction of Conditional Lethal Amber Mutations in Escherichia coli/ Part II Bioinformatics: 22 Statistical Methods for Building Random Transposon Mutagenesis Libraries/ 23 Statistical Evaluation of Genetic Footprinting Data/ 24.Do Essentiality Screens Yield Divergent Results?/ 25 Statistical Analysis of Fitness Data Determined by TAG Hybridization on Microarrays/ 26 Profi ling of Escherichia coli Chromosome Database/ 27 Gene Essentiality Analysis Based on DEG, a Database of Essential Genes/ 28 Detection of Essential Genes in Streptococcus pneumoniae Using Bioinformatics and Allelic Replacement Mutagenesis/ 29 Design and Application of Genome-Scale Reconstructed Metabolic Models/ 30 Predicting Gene Essentiality Using Genome-Scale in Silico Models/ 31 Comparative Approach to Analysis of Gene Essentiality/ Index/ *
Schwartz, E. ed.
Microbial Megaplasmids
Springer-Verlag 2009.3
348 pp.(H)
ISBN 3-540-85466-5
26,600円
Contents
1.Megaplasmids in Archaea/ 2.Megaplasmids and Biodegradation/ 3.Megaplasmids in Plant Symbionts and Pathogens/ 4.Megaplasmids in Human Pathogens/ 5.Megaplasmids and Special Metabolic Functions/ Index/
* Megaplasmids are extrachromosomal genetic elements in the size range of 100 kb and larger. They are found in physiologically and phylogenetically diverse groups of bacteria and archaea. By definition, megaplasmids are not essential for the viability of their hosts under all growth conditions, but paradoxically many megaplasmids carry the genetic information for the defining and characteristic traits of the organism in which they reside.
* Microbial Megaplasmids reviews our knowledge of the extensively studied representatives, such as the catabolic plasmids of the pseudomonads, the rhizobial Sym plasmids, the Ti plasmids of the genus Agrobacterium and the giant enterobacterial virulence plasmids. It also presents snapshots of more recently discovered megaplasmids. The contribution of megaplasmids to the biology of their hosts is described, highlighting the interactions between megaplasmid and chromosomal genes. *
Meneely, P.
Advanced Genetic Analysis
Genes, Genomes, and Networks in Eukaryotes
Oxford U.P. 2009.2
528 pp.(P)
ISBN 0-19-921982-6
8,200円
Contents
* How can we explore gene expression in time and space? How do we know what role a particular gene has? How do some genes control the expression of others? How do genes interact to form gene networks? Advanced Genetic Analysis probes fascinating questions such as these by asking "How can the principles of genetics be used as analytical tools to solve biological problems?"
* With its unique integration of genetics and molecular biology, Advanced Genetic Analysis provides a broad survey of how our understanding of key genetic phenomena can be used to understand biological systems. Opening with a brief overview of key genetic principles and model organisms, the books goes on to explore the use of gene mutations and the analysis of gene expression and activity, before considering the interactions of genes during suppression and epistasis, and how we study gene networks.
* Drawing on the latest experimental tools, including microarrays, RNAi, and bioinformatics approaches, Advanced Genetic Analysis provides a state-of-the-art review of the field, but in a truly student-friendly manner. It uses extended case studies and text boxes to augment the narrative, taking the reader right to the forefront of contemporary research without losing clarity of explanation and insight. *
Johnson, T. R. & Case, C. L.
Laboratory Experiments in Microbiology 9th ed.
微生物学実験法 物９版
Addison-Wesley 2009.1
496 pp.(P)
ISBN 0-321-56028-0
13,600円
Contents
1.Microscopy/ 2.Handling Bacteria/ 3.Staining Methods/ 4.Cultivation of Bacteria/ 5.Microbial Metabolism/ 6.Microbial Growth/ 7.Control of Microbial Growth/ 8.Microbial Genetics/ 9.The Microbial World/ 10.Viruses/ 11.Interaction of Microbe and Host/ 12.Immunology/ 13.Microorganisms and Disease/ 14.Microbiology and the Environment/ Appendices/ Index/
* Containing 57 thoroughly class-tested exercises, this manual provides basic microbiology techniques with applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs.
* The Ninth Edition features a new, four-color design and a dramatically new art program. Many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style, and detailed, colorful photomicrographs that were once grouped together in a color insert are now integrated throughout the exercises. Experiments have been refined throughout, and a new exercise on parasitic helminths provides students with valuable practice in microscopic examination and observation. *
Michiels, C. et al. ed.
High-Pressure Microbiology
ASM Press 2008.3
364 pp.(H)
ISBN 1-55581-423-9
20,100円
Contents
1.High Hydrostatic Pressure Effects in the Biosphere: from Molecules to Microbiology/2.Effects of Hydrostatic Pressure on Viruses/ 3.Effects of High Pressure on Bacterial Spores/ 4.Inactivation of Escherichia coli by High Pressure/ 5.Cellular Impact of Sublethal Pressures on Escherichia coli/ 6.Listeria monocytogenes High Hydrostatic Pressure Resistance and Survival Strategies/ 7.Effects of Pressure on Lactic Acid Bacteria/ 8.Saccharomyces cerevisiae Response to High Hydrostatic Pressure/ 9.Effects of Growth-Permissive Pressures on the Physiology of Saccharomyces cerevisiae/ 10.Factors Affecting Inactivation of Food-Borne Bacteria by High Pressure/ 11.Introduction to Deep-Sea Microbiology/ 12.Isolation, Cultivation, and Diversity of Deep-Sea Piezophiles/ 13.Culture-Independent Characterization of Microbial Diversity in Selected Deep-Sea Sediments/ 14.Deep-Sea Geomicrobiology/ 15.Deep-Sea Fungi/ 16.Physiology and Biochemistry of Methanocaldococcus jannaschii at Elevated Pressures/ 17.Molecular Biology of the Model Piezophile, Shewanella violacea DSS12/ 18.Adaptations of the Psychrotolerant Piezophile Photobacterium profundum Strain SS9/ Index/
* A landmark volume covering the exciting new knowledge on the effects of high pressure on microorganisms *
Yuryev, A. ed.
PCR Primer Design
Humana Press 2007.7
431 pp.(H)
ISBN 1-58829-725-X
10,600円
Contents
Part I. Basic Principles and software for PCR primer design: 1.Physical Principles and Visual-OMP Software for Optimal PCR Design/ 2.Oligo 7 Primer Analysis Software/ 3.Selection for 3' end triplets for polymerase chain reaction primers/ 4. The reference point method in primer design/ 5. PCR primer design using statistical modeling/ 6. Developing a Statistical Model for Primer Design/ Part II. Genome scale PCR primer design: 7. GST-PRIME: An Algorithm for Genome-Wide Primer Design/ 8. Genome-Scale Probe and Primer Design with PRIMEGENS Repeat masking for PCR primer design/ 9. SNPbox: web-based high-throughput primer design with an eye for repetitive sequences/ 10. Fast masking of repeated primer binding sites in eukaryotic genomes Multiplex PCR primer design/ 11. Degenerate Primer Design: Theoretical Analysis and the HYDEN program/ 12. An Iterative Method for Selecting Degenerate Multiplex PCR Primers/ 13. Primer-design for multiplexed genotyping/ 14. MultiPLX: automatic grouping and evaluation of PCR primers/ 15. MultiPrimer: A system for Microarray PCR Primer Design Allele-specific PCR/ 16. Modified oligonucleotides as tools for allele-specific amplification/ 17. AlleleID: A Pathogen Detection and Identification System Long PCR primer design/ 18. GenoFrag, a software package for the design of primers dedicated to whole genome scanning by LR-PCR. DNA methylation mapping/ 19. Designing PCR primer for DNA methylation mapping/ 20. BiSearch: ePCR tool for native or bisulfite treated genomic template/ 21. Graphical design of primers with PerlPrimer/ Index/
* In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. *
Goldman, G. H. & Osmani, S. A.
The Aspergilli
Genomics, Medical Aspects, Biotechnology, and Research Methods
CRC Press 2007.12
576 pp.(H)
ISBN 0-8493-9080-X
18,400円
Contents
1.Basic Biology/ 2.Medically Important Species in the Genus. Biotechnological Aspects of the Genus/ 3.Appendix: Techniques and Resources/ Index/
* With high-quality genome sequences for the important and ubiquitous Aspergilli now available, increased opportunities arise for the further understanding of its gene function, interaction, expression, and evolution. The Aspergilli: Genomics, Medical Aspects, Biotechnology, and Research Methods provides a comprehensive analysis of the research that reveals the main biological attributes of these species. The co-editors are a particularly proficient and prolific pair with long track records of scientific productivity. The book sets the stage with a discussion of basic biology, examining the data on the structure of genomes and comparing the genetic map and annotation methodology. It includes a comparison of metabolic abilities among different Aspergillus spp. and other species, then covers areas such as comparative biology, pathogenic properties, and metabolic capabilities of the Aspergilli. The book reviews established techniques and new methodologies for the post-genomic era in Aspergillus spp. It comes with a CD containing color illustrations to supplement the text. *
273-27 登録日 09.06.28
