Nellen, N. & Hammann, C. ed.
Small RNAs 2nd
Analysis and Regulatory Functions
Springer-Verlag 2008.
219 pp.(P)
ISBN 3-540-74270-0
9,100円
Contents
Part.1:Small Regulatory RNAs in Bacteria: 1. Introduction/ 2. SearchesandDiscoveryofsRNAs/ 3. The Old and New Ones―Biological Functions/ 4. What's Out There in Other Bacteria?/ 5. Mechanisms: Antisense and Protein Sequestration/ 6. ConcludingRemarks/ References/ Part.2:Small Nucleolar RNAs: Identification, Structure, and Function: 1. Introduction/ 2. Experimental IsolationofncRNAs/ 3. ComputationalApproaches toFindncRNAs/ 4. SmallNucleolarRNAs/ 5. SmallCajal-Body-SpecificRNAs/ 6. Protein Association and Function/ 7. GenomicOrganization/ 8. Modification Guide RNAs―Targets and Function/ 9. ConclusionandFuturePerspective/ References/ Part.3: A Computational Approach to Search for Non-Coding RNAs in Large Genomic Data: 1. Introduction/ 2. ProbabilityofPatterns/ 3. DesignandRefinementofPatterns/ 4. SearchingPatterns inLargeSequence Sets/ 5. WWWTools/ References/ Part.4: Experimental Strategies for the Identification and Validation of Target RNAs that Are Regulated bymiRNAs: 1. Introduction/ 2. Post-Transcriptional Inhibition ofmiRNAFunctionbyAntisense Sequences/ 3. Direct Identification of Target mRNA by a PCR Strategy MimickingthemiRNAInteraction/ 4. Validation of miRNA/mRNA Target Pairs bySensorConstructs/ 5. ConcludingRemarks/ References/ Part.5: Protein Interactions with Double-Stranded RNA in Eukaryotic Cells: 1. Introduction/ 2. Occurrence and Origin of Cellular Double-Stranded RNA/ 3. Protein Interactions/ 4. Cellular Response to Double-Stranded RNA/ References/ Part.6: Transitive and Systemic RNA Silencing: Both Involving an RNA Amplification Mechanism?: 1. Introduction/ 2. RNA-Dependent RNA Polymerases and RNA Amplification/ 3. RNA Amplification and Transitive Silencing/ 4. RNAAmplificationandSystemicSilencing/ 5. Absence of Transitive and Systemic RNA Silencing in DrosophilaandMammals/ References/ Part.6: RNA Interference and Antisense Mediated Gene Silencing: 1. Introduction/ 2. Partsof theRNAiMachine/ 3. AntisenseRNAMediatedGene Silencing/ 4. AntisenseRNAandRNAi/ 5. Models/ References/ Part.7: Epigenetic Silencing of Transposons in the Green Alga Chlamydomonas reinhardtii/ 1. Introduction/ 2. Post-TranscriptionalGene Silencing/ 3. TranscriptionalGene Silencing/ 4. ConcludingRemarks/ References/ Part.8: RNA-Dependent Gene Silencing and Epigenetics in Animals: 1. MicroRNAs and Small Interfering RNAs― TwoDifferentTypesofSmallRNAs/ 2. PosttranscriptionalRNAi/ 3. Developmental Gene Regulation by miRNA-Mediated RNAi/ 4. EpigeneticGeneSilencing/ 5. Small dsRNAs May Mediate Epigenetic Silencing of Genes/ 6. Epigenetic Silencing by Antisense Transcripts/ 7. Dose Compensation on Sex Chromosomes/ 8. The Role of roX RNAs in Dose Compensation in Drosophila/ 9. XChromosomeInactivationinMammals/ 10. Conclusions/ References/ Part.9: Potentials of a Ribozyme-Based Gene Discovery System: 1. Introduction/ 2. Randomized Ribozyme Libraries for Gene Discovery/ 3. Identification of Genes Involved in Hepatitis C Virus Internal Ribosome Entry Site Mediated Translation/ 4. Identification of Genes Involved in Cell Transformation/ 5. Identification of Genes Involved in the Pathway of Apoptosis/ 6. Identification of Metastasis-Related Genes/ 7. Identification of Genes Involved in Alzheimer’s Disease/ 8. Conclusions/ References/ Subject Index/
* In recent years Molecular Biology has experienced an unprecedented revolution by the discovery of functional small RNAs. The number of cellular processes in which non-coding RNAs are involved is growing rapidly and include gene regulation on the transcriptional, post-transcriptional and translational level. To complicate matters, these processes seem to be strongly interconnected on the one hand, and diverse among different organisms on the other. This volume describes strategies for the discovery and validation of small RNAs and provides a snapshot of our current understanding of the different mechanisms triggered by small RNAs. *
Appasani, K. ed.
microRNAs
From Basic Science to Disease Biology
Cambridge University Press 2008.2
560 pp.(H)
ISBN 0-521-86598-0
24,000円
Contents
Part.I:Discovery of microRNAs in various organisms: 1.The microRNAs of C. elegans/ 2.Non-coding RNAs - development of man-made vector-based intronic microRNAs (miRNAs)/ 3.Seeing is believing: strategies for studying microRNA expression/ 4.MicroRNAs in limb development/ 5.Identification of miRNAs in the plant Oryza sativa/ Part.II:MicroRNA functions and RNAi-mediated pathways: 6.Inhibition of translation initiation by a microRNA/ 7.In situ analysis of microRNA expression during vertebrate development/ 8.MicroRNA function in the nervous system/ 9.MicroRNA expression that controls the amount of branched chain ?-ketoacid dehydrogenase in mitochondria of human cells/ 10.MicroRNAs and the regulation of leaf shape/ Part.III:Computational biology of microRNAs: 11.miRBase: a database of microRNA sequences, targets and nomenclature/ 12.Computational prediction of microRNA targets in vertebrates, fruitflies and nematodes/ 13.Computational approaches to elucidate miRNA biology/ 14.The RNAhybrid approach to microRNA target prediction/ 15.Machine learning predicts microRNA target sites/ 16.Models of microRNA-target coordination/ Part.IV:Detection and quantitation of microRNAs: 17.Detection and analysis of microRNAs using LNA (locked nucleic acid)-modified probes/ 18.Detection and quantitation of microRNAs using the RNA Invader assay/ 19.A single molecule method to quantify miRNA gene expression/ 20.Real-time quantification of microRNAs by TaqMan assays/ 21.Real-time quantification of miRNAs and mRNAs employing universal reverse transcription/ 22.Dysregulation of microRNAs in human malignancy/ 23.High throughput microRNAs profiling in cancers/ 24.Roles of microRNAs in cancer and development/ 25.miR-122 in mammalian liver/ 26.MiRNAs in glioblastoma/ 27.Role of microRNA pathway in Fragile X mental retardation/ 28.Insertion of miRNA125b-1 into immunoglobulin heavy chain gene locus mediated by V(D)J recombination in precursor B cell acute lymphoblastic leukemia/ 29.miRNAs in TPA-induced differentiation of HL-60 cells/ 30.MiRNAs in skeletal muscle differentiation/ 31.Identification and potential function of viral microRNAs/ 32.Lost in translation: regulation of HIV-1 by microRNAs and a key enzyme of RNA-directed RNA polymerase/ Part.VI:MicroRNAs in stem cell development: 33.MicroRNAs in the stem cells of the mouse blastocyst/ 34.The role of miRNA in hematopoiesis/ 35.MicroRNAs in embryonic stem cell differentiation and prediction of their targets/ 36.Generation of single cell microRNA expression profile/ 37.Piwi-interacting RNAs (piRNAs)/ 38.MicroRNAs in immunology, cardiology, diabetes, and unicellular organisms/ Index/ *
Paddison, P. J. & Vogt, P. K. ed.
RNA Interference
Springer-Verlag 2008.
276 pp.(H)
ISBN 3-540-75156-4
19,400円
Contents
1.RNA interference in mammalian cell systems/ 2.RNAi pathway in C. elegans: The Argonautes and collaborators/ 3.Genetics and biochemistry of RNAi in Drosophila/ 4.Role of Dicer in post-transcriptional RNA silencing/ 5.The mechanism of RNase III action: How Dicer Dices/ 6.microRNA metabolism in plants/ 7.Structure-function relationships among RNA-dependent RNA polymerases/ 8.RNAi-mediated chromatin silencing in fission yeast/ 9.A role for RNAi in heterochromatin formation in Drosophila/ 10.RNA mediated transcriptional gene silencing in human cells/ 11.RNA silencing in mammalian oocytes and early embryos/ 12.Identifying human MicroRNAs/ Subject index/ *
Sanderson, M. R. & Skelly, J. V. ed.
Macromolecular Crystallography
conventional and high-throughput methods
Oxford U.P. 2007.9
292 pp. (H)
ISBN 0-19-852097-2
17,800円
Contents
1. Classical Cloning, Expression and Purification / 2. High-Throughput Cloning, Expression and Purification / 3. Automation of Non-Conventional Crystallization Techniques for Screening and Optimisation / 4. First Analysis of Macromolecular Crystals / 5. In-House Macromolecular Data Collection / 6. Solving the Phase Problem Using Isomorphous Replacement / 7. Molecular Replacement Techniques for High-Throughput Structure Determination / 8. MAD Phasing / 9. Application of Direct Methods to Macromolecular Structure Solution / 10. Phase Refinement through Density Modification / 11. Getting a Macromolecular Model: Model Building, Refinement and Validation / 12. High-Throughput Crystallographic Data Collection at Synchrotrons / 13. Electron Density Fitting and Structure Validation / 14. RNA Crystallogenesis / 15. Crystallography in the Study of Protein-DNA Interaction / 16. Virus Crystallography / 17. Macromolecular Crystallography in Drug Design / *
* Macromolecular Crystallography is the study of macromolecules (proteins and nucleic acids) using X-ray crystallographic techniques in order to determine their molecular structure. *
Torchilin, V. P. ed.
Delivery of Protein and Peptide Drugs in Cancer
Imperial College Press 2006.4
392 pp. (H)
ISBN 1-86094-627-5
13,800円
Contents
1. Influence of Tumor Physiology on Delivery of Therapeutics / 2. Enhanced Permeability and Retention (EPR) Effect and Tumor-Selective Delivery of Anticancer Drugs / 3. Basic Strategies for PEGylation of Peptide and Protein Drugs / 4. PEGylated Proteins as Cancer Therapeutics / 5. PEGylated Proteins in Immunotherapy of Cancer / 6. Silencing Proteins: Nanotechnological Approaches to Deliver of siRNA for Cancer Therapy / 7. Anti-Cancer Proteins and Peptides in Liposomes / 8. Folate-Mediated Delivery of Protein and Peptide Drugs into Tumors / 9. Transferrin Receptor Mediated Delivery of Protein and Peptide Drugs into Tumors / 10. Transmembrane Delivery of Protein and Peptide Drugs into Cancer Cells / 11. Protein and Peptide Drugs to Suppress Tumor Angiogenesis / 12. Utilizing Lymphatic Transport in Enhancing the Delivery of Drugs, Including Proteins, and Peptides, to Metastatic Tumors / 13. Delivery of Protein and Peptide Drugs to Brain Tumors / 14. Protein and Peptide-Based Cancer Gene Therapy / *
Howe, C.
Gene Cloning and Manipulation 2nd ed.
Cambridge University Press 2007.8
276 pp. (P)
ISBN 0-521-52105-x
7,100円
Contents
Preface; 1. The tools for the job; 2. Polymerase chain reaction; 3. Simple cloning; 4. Other vector systems for E. Coli; 5. Making libraries; 6. Screening libraries; 7. Modification and mutagenesis; 8. Use of cloned DNA; 9. Using other organisms; 10. Examples. *
* Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. *
Bringmann, P. et al.
Systems Biology
Applications and Perspectives
Springer-Verlag 2007.1
172 pp. (H)
ISBN 3-540-31338-9
9,800円
Contents
1 RNAi Applications in Target Validation / 2 Does the Serum Peptidome Reveal Hemostatic Dysregulation? / 3 Systems Biology: New Paradigms for Cell Biology and Drug Design / 4 The Theory of Biological Robustness and Its Implication in Cancer / 5 Network Genomics / 6 A Plea for More Theory in Molecular Biology / 7 Applying a Causal Framework to System Modeling / 8 Can Cell Systems Biology Rescue Drug Discovery? / Previous Volumes Published in This Series / *
* This volume features contributions from participants of an ESRF Workshop on "Systems Biology" held in Berkeley, USA, in November 2005. *
896-16 登録日 08.03.22
