Wang, Z. & Yang, B.
MicroRNA Expression Detection Methods
Springer-Verlag 2010.2
387 pp.(H)
ISBN 3-642-04927-3
22,500円
Contents
1. General Remarks/ 2. miRNA Microarray Methods/ 3. Northern Blotting Methods/ 4. In Situ Hybridization Methods/ 5. Real-Time RT-PCR Methods/ 6. Cloning Methods/ 7. Nanoparticle Methods/ 8. Other Methods/ 9. Circulating miRNA Detection Methods/ 10. Single-cell miRNA Detection Methods/ 11. Whole Mount In Situ Analysis/ References/ Index/
* This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression, their application in miRNA research and their potential as tools for clinical diagnosis and prognosis. *
Reidhaar-Olson, J. F. & Rondinone, C. M.
Therapeutic Applications of RNAi
Methods and Protocols
Springer-Verlag 2009.7
143 pp.(H)
ISBN 1-60327-294-1
12,000円
Contents
1. Development and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in Tumors/ 2. Targeted Gene Silencing into Solid Tumors with Electrically Mediated siRNA Delivery/ 3. Liposomal siRNA for Ovarian Cancer/ 4. Therapeutic Applications of RNAi for Silencing Virus Replication/ 5. RNAi in the Malaria Vector, 6. RNAi Using a Chitosan/siRNA Nanoparticle System: in vitro and in vivo Applications/ 7. Lentiviral and Adeno-Associated Vector-Based Therapy for Motor Neuron Disease through RNAi/ 8. Studying Autoimmunity by in vivo RNA Interference/ 9. Transcriptional Gene Silencing Using Small RNAs/ 10. Alternative Splicing as a Therapeutic Target for Human Diseases/ Index/ *
Gusev, Y. ed.
MicroRNA Profiling in Cancer
A Bioinformatics Perspective
World Scientific Publishing Co.Pte. Ltd. 2009.10
272 pp.(H)
ISBN 981-4267-01-5
15,800円
Contents
1. Measurement and Interpretation of MicroRNA Expression Profiles/ 2. MicroRNA Expression Analysis by LNA Enhanced Microarrays/ 3. Analysis and QC for Real-Time QPCR Arrays of Human MicroRNAs/ 4. Massively Parallel MicroRNA Profiling in the Haematologic Malignancies/ 5. Who Dunit? MicroRNAs Involved in Prostate Cancer/ 6. Intronic MicroRNA: Creation, Evolution and Regulation/ 7. MiRome Architecture and Genomic Instability/ 8. Interconnection of MicroRNA and Transcription Factor-Mediated Regulatory Networks/ 9. Analysis of MicroRNA Profiling Data with Systems Biology Tools/ 10. Mathematical and Computational Modeling of Post-Transcriptional Gene Regulation by MicroRNAs/ Index/
* This volume contains adaptations and critical reviews of the recent state-of-the-art studies, ranging from technological advances in microRNA detection and profiling, clinically oriented microRNA profiling in several human cancers, to a systems biology analysis of global patterns of microRNA regulation of signaling and metabolic pathways. Interactions with transcription factor regulatory networks and mathematical modeling of miRNA regulation are also discussed. *
Herschlag , D. ed.
Biophysical, Chemical, and Functional Probes of RNA Structure,
Interactions and Folding Part A
(Methods in Enzymology Vol.468)
RNA構造と折り畳への生物物理的アプローチ
Academic Press 2009.11
496 pp.(H)
ISBN 0-12-374399-0
19,400円
Contents
1. Equilibrium hydroxyl radical footprinting/ 2. Bench-top time-resolved hydroxyl radical footprinting/ 3. Analysis of hydroxyl radical footprinting gels 'SAFA'/ 4. Kinetic modeling of reaction pathways from hydroxyl radical data/ 5. "BABE" mapping of protein/RNA position/ 6. Multiplexed?.(MOHCA)/ 7. SHAPE/ 8. "In-line Probing" / 9. NAIM/ 10. Other NAIM/ 11. Purification of T7 RNA Polymerase/ 12. Purification of T4 RNA ligase/ 13. In vitro transcription of RNA/ 14. 32P-labeling of RNA/ 15. Fluorescent labeling of RNAs/ 16. Assembly of complex RNAs by �]Moore-Sharp' ligations/ 17. Assembly of complex RNAs by �]Moore-Sharp' ligations/ 18. General considerations for smFRET with RNA samples/ 19. Ion counting/ 20. ASAXS/ 21. NLPB/ 22. smNLPB/ 23. Gel mobility mapping of junction structure/ 24. Temperature gradient gels/ 25. Melting studies/ 26. Co-transcriptional folding studies/ 27. Activity assays to follow folding processes/ 28. 2AP fluorescence/ 29. EPR measurements of RNA dynamics/ 30. EPR measurements of RNA dynamics/ 31. FPA measurements of RNA dynamics/ 32. EPR methods to study specific metal ion binding sites in RNA/ 33. Thermodynamic study of site-specific metal ion binding sites in RNA/ 34. Oligonucleotide hybridization studies of RNA folding pathways/ 35. Native gel shifts/ 36. Tiling arrays to assess RNA structure/ 37. EPR distance measurements in RNA/ 38. RNA folding in vivo/ 39. Cleavage of RNAs with �]restriction DNAzymes'/ Index/
* This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells. *
Endo, Y. et al. ed.
Cell-Free Protein Production
Methods and Protocols
Humana Press 2009.12
264 pp.(H)
ISBN 1-60327-330-1
12,800円
Contents
1. Cell-Free Protein Preparation through Prokaryotic Transcription-Translation Methods/ 2. PURE Technology/ 3. The Cell-Free Protein Synthesis System from Wheat Germ/ 4. A Cell-Free Protein Synthesis System from Insect Cells/ 5. Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells/ 6. Analysis of Protein Functions through a Bacterial Cell-Free Protein Expression System/ 7. Cell-Free Based Protein Microarray Technology Using Agarose/DNA Microplate/ 8. An Efficient Approach to the Production of Vaccines Against the Malaria Parasite/ 9. Protein Engineering Accelerated by Cell-Free Technology/ 10. Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds/ 11. NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System/ 12. Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination/ 13. Cell-Free Protein Synthesis for Structure Determination by X-Ray Crystallography/ 14. Production of Multi-Subunit Complexes on Liposome through an E. coli Cell-Free Expression System/ 15. Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System/ 16. Strategies for the Cell-Free Expression of Membrane Proteins/ 17. Production of Membrane Proteins through the Wheat-Germ Cell-Free Technology/ 18. Ribosome Display with the PURE Technology/ 19. Incorporation of 3-azidotyrosine into Proteins through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification/ 20. Synthesis of Functional Proteins within Liposomes/ Index/
* During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. *
Atkins, J. F. & Gesteland, R. F. ed.
Recoding
Expansion of Decoding Rules Enriches Gene Expression
Springer-Verlag 2009.12
466 pp.(H)
ISBN 0-387-89381-4
24,000円
Contents
1. Redefinition/ 2. Frameshifting - Redirection of Linear Readout/ 3. Discontiguity/ 4. Transcription Slippage/ 5. Appendix/ Index/
* The dynamic nature of decoding the information in messenger RNA was unanticipated at the time the genetic code was first deciphered. We now know that both the meaning of individual codons and the framing of the readout process can be modified by information in specific messenger RNAs.
* This book describes this "Recoding" phenomenon, revealing the diversity of an additional layer of information in mRNAs that serves to enrich the expression of genes. Knowledge of recoding is essential for understanding the organization and expression of genes in viruses and all organisms ranging from bacteria to archeae to plants to humans, making Recoding pertinent to all biological sciences. *
Cassimeris, L.
Lewin's CELLS 2nd ed.
ルーウィンの細胞学 第２版
Jones & Bartlett (A) 2010.3
1056 pp.(H)
ISBN 0-7637-6664-X
18,500円
Contents
1. Introduction/ 2. Membranes and Transport Mechanisms/ 3. The Nucleus/ 4. The Cytoskeleton/ 5. Cell Division, Apoptosis, and Cancer/ 6. Cell Communication/ 7. Prokaryotic and Plant Cells/ Index/
* Redesigned to incorporate new learning tools and elements, this edition continues to provide readers with current coverage of the structure, organization, growth, regulation, movements, and interaction of cells, with an emphasis on eukaryotic cells.
* Under the direction of three expert lead editors, new chapters on metabolism and general molecular biology have been added by subject specialist. All chapters have been carefully edited to maintain consistent use of terminology and to achieve a homogenous level of detail and rigor. A new design incorporates many new pedagogical elements, including Concept & Reasoning Questions, Methods boxes, Clinical Applications boxes, and more. *
896-24 登録日 10.04.04
