Hagen, J. V. ed.
Proteomics Sample Preparation
プロテオミクス試料調整
Wiley -VCH 2008.5
485 pp.(H)
ISBN 3-527-31796-1
15,900円
Contents
1.Perspectives in Proteomics Sample Preparation/ 2.Mass Spectrometry/ 3.Electrophoresis/ 4.Optical Methods/ 5.Chromatogaphy/ 6.Structural Proteomics/ 7.Interaction Analysis/ 8.Post-Translational Modifications/ 9.The Human Proteosome/ 10.Bioinformatics/ Index/
* This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task. *
Westermeier, R. et al.
Proteomics in Practice 2nd ed.
A Guide to Successful Experimental Design
Wiley -VCH 2008.5
502 pp.(H)
ISBN 3-527-31941-7
16,000円
Contents
1.Proteomics Technology/ 2.Practical Manual of Proteome Analysis/ 3.Trouble Shooting/ References Index
* A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries.
* The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third section is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further detail. *
Sussman, J. L. & Silman, I. ed.
Structural Proteomics and Its Impact on the Life Sciences
World Scientific Publishing Co.Pte. Ltd. 2008.6
600 pp.(H)
ISBN 981-277-204-9
19,800円
Contents
1. The Importance of Target Selection Strategies in Structural Biology/ 2. The Impact of Structural Proteomics on Macromolecular Structure Databases/ 3. The Impact of 3D Structures on a Protein Knowledgebase: From Proteins to Systems/ 4. Bioinformatics of Protein Function/ 5. Comparative Modeling in Structural Genomics/ 6. The Contribution of Structural Proteomics to Understanding the Function of Hypothetical Proteins/ 7. Intrinsically Disordered Proteins/ 8. Metalloproteins: Structure, Conservation and Prediction of Metal Binding Sites/ 9. The Impact of Protein Expression Methodologies on Structural Proteomics/ 10. Protein Complexes Assembly by Multi-Expression in Bacterial and Eukaryotic Hosts/ 11. The Impact of Structural Proteomics on the Prediction of Protein-Protein Interactions/ 12. Cryo-Electron Microscopy in the Era of Structural Proteomics/ 13. On NMR-based Structural Proteomics/ 14. Structural Proteomics in Relation to Signaling Pathways/ 15. The Impact of Structural Proteomics and Drug Design/ 16. Structural Proteomics of Emerging Viruses: The Example of SARS-CoV and Other Coronaviruses/ 17. High-throughput Technologies for Structural Biology: The Protein Structure Initiative Perspective/ 18. European Structural Proteomics A Perspective/ 19. Structural Genomics and Structural Proteomics: A Global Perspective/ 20. Policies in Structural Genomics/Structural Proteomics/ Index/ *
Link, A. J. at al.
Proteomics
A Cold Spring Harbor Laboratory Course Manual
（プロテオミクス： CSHL実験講義マニュアル）
Cold Spring Harbor Laboratory 2008.6
200 pp.(H)
ISBN 0-87969-793-8
18,900円
Contents
* 本書はCold Spring Harbor Laboratoryで開催されている講義をもとに、最新機器を用いたプロテオミクスの実験法をまとめたマニュアルです。
* 最新プロトコルや実験上のヒント、講義資料を収録しており、幅広い研究分野の研究者に役立つようまとめられています。本書には詳細なプロトコルが多数収録され、タンパク質マイクロアレイ、液体クロマトグラフィー、発現構築体、IMAC、質量分析法、MALDI-TOF、MudPITなどの実験手順が詳しい図解付で詳しく解説され、参考文献やウェブサイトのリストも掲載されています。
Table of Contents:
1. 2-D Dimensional Gel Electrophoresis Analysis of Whole Cell Lysates/ 2. Purification of Protein Complexes Using Tandem Affinity Purification (TAP) for Mass Spectrometry Analysis/ 3. Matrix Assisted Laser Desorption Ionization and Time of Flight Mass Spectrometry Analysis of Trypsin-digested Proteins (MALDI-TOF/TOF)/ 4. Liquid Chromatography and Tandem Mass Spectrometry Analysis of Trypsin-digested Proteins (LC-MS/MS)/ 5. Phosphopeptide Analysis Using IMAC and Mass Spectrometry/ 6. Multidimensional Protein Identification Technology (MUDPIT) Analysis of Whole Cell Lysates/ 7. Quantitative Mass Spectrometry Analysis of Whole Cell Extracts (iTRAQ)/ 8. High-throughput Cloning of ORFs (Assembling Large Sets of Expression Constructs)/ 9. Construction of Protein Microarrays (Nucleic Acid-Programmable Protein Array (NAPPA)/ 10. Detecting Protein-protein Interactions Using Protein Microarrays/ Appendices: Preparation of Glass Slides for NAPPA/ Quantifying Expression Clone DNA for NAPPA/ Microarraying Expression Clones onto Glass Slides for NAPPA/ Setup of an Electrospray Ionization Source/ In-gel Digest/ Solution Protein Digest/ TCA Precipitation of Proteins/ Average and Monoisotopic Masses of Amino Acids/ Dipeptide Masses of Amino Acids/ Recipes of Solutions/ Recipes of Media/ Tune File Setup for LTQ/ LTQ MS Methods/ Offline Desalting/ Index/ *
939-11 登録日 08.06.30
